Doctorat, Université de Toulouse, 1991
HDR, Université de Poitiers, 2000

Responsabilités

• Directeur de l’Unité – UMR7267
• Membre de la section 20 du Comité national du CNRS
• Membre du conseil de l’UFR Sciences Fondamentales et Appliquées de l’Université de Poitiers

Thèmes de Recherche

Isolement et caractérisation de molécules anti-Legionella et étude du mode d’action de ces agents antimicrobiens (peptides et biosurfactants) produits par des microorganismes, afin de les utiliser pour contrôler le développement de Legionella dans l’eau.

Fonctionnalisation de surfaces par greffage, covalent ou non, de molécules antimicrobiennes, dont des peptides, afin d’essayer d’obtenir des systèmes stables pour l’élaboration de matériaux, biomédicaux en particulier, à activité anti-biofilm.

Etude du dialogue moléculaire entre microorganismes, en particulier dans les biofilms (quorum sensing), et son impact dans la prolifération et la virulence des bactéries pathogènes.

Impact de polluants environnementaux, dont des perturbateurs endocriniens (PEs), et de leurs métabolites sur la survie et la virulence de constituants bactériens, commensaux et pathogènes, de divers microbiotes humains (cutané, pulmonaire et intestinal) ou environnementaux.

Mots-clés

Legionella, biofilm, antimicrobien, peptide, quorum sensing, microbiotes, virulence bactérienne

Publications représentatives

1. Gharbi, T. Legigan, V. Humblot, S. Papot and J.M. Berjeaud. « Surface functionalization by covalent immobilization of an innovative carvacrol derivative to avoid fungal biofilm formation. » Sous presse dans AMB express, 2015, 5:9
2. Schlusselhuber, V. Humblot, S. Casale, C. Méthivier, J. Verdon. M. Leippe and J.M. Berjeaud « Potent antimicrobial peptides against Legionella pneumophila and its environmental host, Acanthamoeba castellanii« , Appl. Microbiol. Biotechnol., 2015, 99, 4879–4891.
3. Loiseau, M. Schlusselhuber, R. Bigot, J. Bertaux, J.M. Berjeaud and J. Verdon. “Surfactin from Bacillus subtilis displays an unexpected anti-Legionella activity”, Microbiol. Biotechnol., 2015, 99, 5083–5093
4. Marchand, J. Augenstreich, C. Loiseau, J. Verdon, S. Lecomte and J. M. Berjeaud. « Effect of amino acid substitution in the staphylococcal peptides warnericin RK and PSMa on their anti-Legionella and hemolytic activities”, Cell. Biochem., 2015, 405, 159–167.
5. J. M. Berjeaud, S. Chevalier, M. Schlusselhuber, E. Portier, C. Loiseau, W. Aucher, O. Lesouhaitier and J. Verdon. « Legionella pneumophila: the paradox of a highly sensitive opportunistic waterborne pathogen able to persist in the environment”; Front. Microbiol., 2016, 7, 486,
6. Loiseau, J. Augenstreich, A. Marchand, E. Harté, M. Garcia, J. Verdon, M. Mesnil, S. Lecomte, J.M. Berjeaud^.. “Specific anti-leukemic activity of the peptide Warnericin RK and analogues and visualization of their effect on cancer cells by chemical Raman imaging”, 2016, PLoS One, 11(9):e0162007.

7. Roblin G., Octave S., Faucher M., Fleurat-Lessard P., Berjeaud J.-M. (2018). Cysteine: A multifaceted amino acid involved in signaling, plant resistance and antifungal development. Plant Physiol Biochem 129: 77–89.
8. Corre M.-H., Delafont V., Legrand A., Berjeaud J.-M., Verdon J. (2019). Exploiting the Richness of Environmental Waterborne Bacteria Species to Find Natural Legionella pneumophila Competitors. Front Microbiol 9: 3360.
9. Roblin G., Luini E., Fleurat-Lessard P., Larignon P., Berjeaud J.-M. (2019). Towards a preventive and/or curative treatment of esca in grapevine trunk disease: General basis in the elaboration of treatments to control plant pathogen attacks. Crop Prot 116: 156–169
10. Crepin A., Jegou J-F., Andre S., Ecale F., Croitoru A., Cantereau A., Berjeaud J.-M., Ladram A., Verdon J. (2020). In vitro and intracellular activities of frog skin temporins against Legionella pneumophila and its eukaryotic hosts. Sci Rep 10(1):3978.

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Main responsabilities:

• Director of the Unit – UMR7267
• Member of Section 20 of the NSS National Committee
• Member of the board of the UFR Sciences Fondamentales et Appliquées of the University of Poitiers

Research Topics

Isolation and characterization of anti-Legionella molecules and study of the mode of action of these antimicrobial agents (peptides and biosurfactants) produced by microorganisms, in order to use them to control the development of Legionella in water.

Functionalization of surfaces by grafting, covalent or not, of antimicrobial molecules, including peptides, in order to try to obtain stable systems for the development of materials, biomedical in particular, with anti-biofilm activity.

Study of the molecular dialogue between microorganisms, in particular in biofilms (quorum sensing), and its impact in the proliferation and virulence of pathogenic bacteria.

Impact of environmental pollutants, including endocrine disruptors (EDCs), and their metabolites on the survival and virulence of bacterial, commensal and pathogenic constituents of various human (skin, lung and intestinal) or environmental microbiotes.

Keywords (5)

Legionella, biofilm, antimicrobial, peptide, sensing quorum, microbiotes, bacterial virulence