• Technicien

Compétences :

Extraction ADN, ARN, réalisation de clonage, PCR, Southern et Northern blot…

Extraction de vésicules microsomales et plasmalemmiques sur Arabidopsis Thaliana et levures Saccharomyces Cerevisiae

Absorption de radioactivité par les levures Saccharomyces Cerevisiae et assimilation de CO2  radiomarqué au carbone 14 sur plante entière

Dosage de sucres par réaction enzymatique

Expérimentations végétales

Personne Compétente en Radioprotection

Publications :

Durand, M., Porcheron, B., Hennion, N., Maurousset, L., Lemoine, R., & Pourtau, N. (2016). Water deficit enhances C export to the roots in A. thaliana plants with contribution of sucrose transporters in both shoot and roots. Plant Physiology. doi: 10.1104/pp.15.01926

Wu, H., S. Marhadour, Z. W. Lei, E. Dugaro, C. Gaillard, B. Porcheron, C. Marivingt-Mounir, R. Lemoine, J. F. Chollet and J. L. Bonnemain (2017). « Use of D-glucose-fenpiclonil conjugate as a potent and specific inhibitor of sucrose carriers. » Journal of Experimental Botany 68(20): 5599-5613.611.

Durand, M., Mainson, D., Porcheron, B., Maurousset, L., Lemoine, R., & Pourtau, N. (2018). Carbon source–sink relationship in Arabidopsis thaliana: the role of sucrose transporters. [journal article]. Planta, 247(3), 587-611. doi: 10.1007/s00425-017-2807-4

N’Guyen GQ, Raulo R, Marchi M, Agustí-Brisach C, Iacomi B, Pelletier S, Renou J-P, Bataillé-Simoneau N, Campion C, Bastide F, Hamon B, Mouchès C, Porcheron B, Lemoine R,Kwasiborski A, Simoneau P and Guillemette T (2019) Responses to Hydric Stress in the Seed-BorneNecrotrophic Fungus Alternaria brassicicola.Front. Microbiol. 10:1969 doi:10.3389/fmicb.2019.01969
Anne Mercier, Laure Mignerot, Nils Hennion, Kévin Gravouil, Benoît Porcheron, Mickaël Durand, Laurence Maurousset, Yann Héchard, Joanne Bertaux, Thierry Ferreira, Béatrice Lauga, Rémi Lemoine, Nathalie Pourtau (2021) Growth of Arabidopsis thaliana in rhizobox culture system
evaluated through the lens of root microbiome
Plant and Soil volume 455, pages 467–487 (2020)

Livre: Titre: An experimental rhizobox system for the integrative analysis of root development and abiotic stress responses under water deficit conditions

Running Head: Integrative study of root development and stress responses. Auteur(s): Mickaël Durand1,2…, Amélie1… Morin, Benoit Porcheron1, Nathalie Pourtau1é. Institutions addresses: (1) Écologie et Biologie des Intéractions (EBI) Université de Poitiers, CNRS, EBI, Poitiers, France. (2) present address: EA2106 “Biomolécules et Biotechnologies Végétales”, Université de Tours, Tours 37200, France. (…) These authors have contributed equally to this work and share the first authorship. (é) Correponding author. Key words: Arabidopsis Thaliana, drought, environmental constraints, Pisum Sativum, plant-soil system, rhizobox, roots, water stress.

Morin, M., Porcheron, B., Kodjovi, GC., Moumen,B., Vriet, C., Maurousset,L., Lemoine, R., Pourtau, N., Doidy, J (2023) Genome-wide transcriptional responses to water deficit during seed development in Pisum sativum, focusing on sugar transport and metabolism. Physiologia Plantarum doi: 10.1111/ppl.14062

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